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Construction of chromosomal segment substitution lines and genetic dissection of introgressed segments associated with yield determination in the parents of a super‐hybrid rice
Author(s) -
Liu Xi,
Zhao Zhigang,
Liu Linglong,
Xiao Yinghui,
Tian Yunlu,
Liu Shijia,
Chen Liangming,
Wang Yihua,
Liu Yuqiang,
Chen Saihua,
Zhang Wenwei,
Wang Chunming,
Jiang Ling,
Wan Jianmin
Publication year - 2016
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12329
Subject(s) - panicle , biology , quantitative trait locus , hybrid , inbred strain , heterosis , oryza sativa , agronomy , genetics , horticulture , gene
Heterosis is a phenomenon whereby hybrids of inbred lines produce favourable phenotypes that exceed those of their parents. Traits of interest are higher yield and stronger stress tolerance. The two‐line super‐hybrid rice ‘Liangyoupei9’ ( LYP 9 ) shows superiority to both its elite inbred line ‘93‐11’ and ‘Pei'ai64s’ (‘ PA 64s’) parents and conventional hybrids. However, the genetic basis of its hybrid vigour, especially yield determination, remains elusive. In the present study, a set of 156 chromosome segment substitution lines ( CSSL s) carrying overlapping segments from ‘ PA 64s’ in a genetic background of ‘93‐11’ were constructed and planted in six environments. Three major agronomic traits, viz. panicle length ( PL ), heading date ( HD ) and plant height ( PH ), and five yield‐related traits, viz. grain weight per panicle ( GWP ), number of grains per panicle ( GPP ), 1000‐grain weight ( TGW ), seed set ( SS ) and number of panicles of per plant ( PPP ), were evaluated over 3 years. Quantitative trait loci ( QTL ) analysis was conducted using a likelihood ratio test based on stepwise regression. Forty‐six putative QTL distributed on 11 chromosomes were detected in more than one year. Remarkably, GWP of four CSSL s carrying positive yield QTL outperformed the recurrent parent ‘93‐11’ by more than 15%, in at least two environments. These results indicate that CSSL s are effective in identifying yield‐associated traits, and lines harbouring such QTL will be rich in resources for future molecular breeding programmes.