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Genetic analysis and identification of two soybean mosaic virus resistance genes in soybean [ Glycine max (L.) Merr]
Author(s) -
Li Kai,
Ren Rui,
Adhimoolam Karthikeyan,
Gao Le,
Yuan Yuan,
Liu Zhitao,
Zhong Yongkun,
Zhi Haijian
Publication year - 2015
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12315
Subject(s) - soybean mosaic virus , biology , bulked segregant analysis , genetics , gene , candidate gene , cloning (programming) , cultivar , gene mapping , virus , plant virus , chromosome , potyvirus , botany , computer science , programming language
Soybean mosaic virus ( SMV ) commonly affects soybean production worldwide, and the SC 18 strain has been widespread in C hina. This study aimed to characterize and map the SC 18 resistance genes present in soybean cultivars ‘ K efeng N o. 1’ and ‘ Q ihuang 22’. Inheritance analysis revealed that two independent single dominant genes in K efeng No. 1 and Q ihuang 22 confer resistance to SC 18. Using simple sequence repeat ( SSR ) markers and bulked segregant analysis, the K efeng No. 1 and Q ihuang 22 resistance genes were located on soybean chromosomes 2 and 13, respectively. We further screened two populations of recombinant inbred lines with 32 SSR markers in the target region, where the resistance gene in K efeng No. 1 was fine mapped to an 80‐kb region containing six putative genes. Sequence and expression analyses of these genes revealed that SMV resistance in K efeng No. 1 was probably attributable to three of the candidate genes (i.e. Glyma.02G127800 , Glyma.02G128200 and Glyma.02G128300 ). Collectively, the results of this study will greatly facilitate the cloning of SC 18 resistance genes and marker‐assisted breeding of SMV ‐resistant soybean cultivars.