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Association mapping and resistant alleles’ analysis for japonica rice blast resistance
Author(s) -
Guo Liying,
Guo Wei,
Zhao Hongwei,
Wang Jingguo,
Liu Hualong,
Sun Jian,
Zheng Hongliang,
Sha Hanjing,
Zou Detang
Publication year - 2015
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12310
Subject(s) - biology , japonica , association mapping , allele , marker assisted selection , quantitative trait locus , genetics , cultivar , oryza sativa , gene mapping , genotype , genetic marker , genetic linkage , horticulture , botany , gene , single nucleotide polymorphism , chromosome
Abstract Rice blast is one of the major fungal diseases that badly reduce rice production in C hina and worldwide. Association mapping for blast resistance was performed on 226 japonica rice cultivars with 118 pairs of SSR markers. The blast resistance was evaluated by inoculating with two isolates, DB 22 and DB 77, at the tillering stage in 2013 and 2014, separately. A total of 31 associations with 17 different SSR s were significantly (P < 0.05) associated with blast resistance based on the mixed linear model ( MLM ), of which nine markers could be detected in both 2013 and 2014, including two markers that were simultaneously associated with the two isolates. Five of the nine stable markers were consistent with the genome regions identified by linkage mapping in previous reports. Phenotypic effects of each allele of the nine stable markers were compared, and 18 favourable alleles were identified. Five elite parental combinations were designed for improving blast resistance in rice. Our results demonstrate that association mapping can complement and enhance previous QTL information for marker‐assisted selection and breeding by design.