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Association analysis of five candidate genes with plant height and dry matter yield in perennial ryegrass
Author(s) -
Statkevičiūté Gražina,
Aleliūnas Andrius,
Kemešyté Vilma,
Pašakinskiené Izolda,
Lübberstedt Thomas,
Brazauskas Gintaras
Publication year - 2015
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12280
Subject(s) - biology , indel , perennial plant , genetics , allele , single nucleotide polymorphism , gene , quantitative trait locus , candidate gene , genotype , botany
Five candidate genes Lp IAA 1 , Lp RUB 1 , Lp BRI 1 , Lp SHOOT 1 and Lp TB 1 with putative function in plant architecture were allele sequenced in a panel of 96 diploid perennial ryegrass genotypes of diverse origin. The total length of the non‐redundant genomic DNA alignment was 5425 bp and revealed 270 polymorphic sites in total. A negative significant Tajima's D value was detected in Lp TB 1 gene, suggesting selection pressure for low‐frequency alleles in this gene. All 96 genotypes were evaluated for plant height and dry matter yield over two years. Marker–trait associations were calculated between polymorphic sites and both phenotypic traits. Three indels and three single nucleotide substitutions in Lp TB 1 gene were significantly (P < 0.05, q < 0.05) associated with plant height, while one indel was associated with dry matter yield. The results suggest putative role of Lp TB 1 gene in plant height determination in perennial ryegrass and provide means for target allele selection.