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Fine mapping of qSS ‐9 , a major and stable quantitative trait locus, for seed storability in rice ( Oryza sativa L.)
Author(s) -
Lin Qiuyun,
Jiang Yimei,
Sun Ailing,
Cao Penghui,
Li Linfang,
Liu Xi,
Tian Yunlu,
He Jun,
Liu Shijia,
Chen Liangming,
Jiang Ling
Publication year - 2015
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12264
Subject(s) - oryza sativa , quantitative trait locus , locus (genetics) , backcrossing , biology , japonica , population , allele , chromosome , indel , genetics , botany , gene , demography , sociology , genotype , single nucleotide polymorphism
Seed storability in rice ( Oryza sativa L.) is an important agronomic trait. We previously showed a quantitative trait locus of seed storability, qSS ‐9 , on chromosome 9 in a backcross population of ‘Koshihikari’ ( japonica ) / ‘Kasalath’ ( indica ) // ‘Koshihikari’. In this study, fine mapping of the chromosomal location of qSS ‐9 was performed. Effect of ‘Kasalath’ allele of qSS ‐9 was validated using a chromosome segment substitution line, SL 36, which harboured the target quantitative trait loci ( QTL ) from ‘Kasalath’ in the genetic background of ‘Nipponbare’ under different ageing treatments in different environments. Subsequently, an F 2 population from a cross between ‘Nipponbare’ and SL 36 was used for fine mapping of qSS ‐9 . Simultaneously, four subnear isogenic lines (sub‐ NIL s) that represented different recombination breakpoints across the qSS ‐9 region were developed from F 3 progeny. Finally, the qSS ‐9 locus was located between the Indel markers Y10 and Y13, which delimit a region of 147 kb in the ‘Nipponbare’ genome. These results provide a springboard for map‐based cloning of qSS ‐9 and possibilities for breeding rice varieties with strong seed storability.