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Identification of marker alleles linked to fire blight resistance QTLs in apple genotypes
Author(s) -
Papp David,
Békefi Zsuzsanna,
Balotai Boglárka,
Tóth Magdolna
Publication year - 2015
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12258
Subject(s) - fire blight , biology , cultivar , quantitative trait locus , malus , genotype , allele , blight , genetic marker , marker assisted selection , horticulture , molecular marker , genetics , gene , erwinia
Molecular marker analysis can be an effective tool when searching for new fire blight resistance donors. It can speed up the breeding process as well, even though many of the available markers linked to fire blight resistance QTL s have not yet been tested by screening a large number of cultivars. The aim of this study was to search for alternate sources of the three major QTL s of fire blight resistance; FBF 7, FB _ MR 5 and FB _E, as well as to test the efficiency of some markers linked to minor QTL s. Altogether, nine primer pairs were used on 77 genotypes including new Hungarian cultivars and old apple cultivars from the Carpathian basin. Several marker alleles of FB resistance QTL s have been detected in the screened genotypes, most importantly the alleles coupling with FB _ MR 5 in the old cultivars ‘Kéresi muskotály’, ‘Szabadkai szercsika’ and ‘Batul’. We propose these cultivars as the first available resistance donors of FB _ MR 5 instead of the crabapple Malus × robusta 5. The results also bring new information regarding the resistance alleles of new Hungarian cultivars and selections.