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Chromosomal regions associated with the in vitro culture response of wheat ( T riticum aestivum L .) microspores
Author(s) -
Nielsen Nanna H.,
Andersen Stig U.,
Stougaard Jens,
Jensen Anni,
Backes Gunter,
Jahoor Ahmed
Publication year - 2015
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12257
Subject(s) - doubled haploidy , biology , quantitative trait locus , plantlet , microspore , germplasm , population , hybrid , ploidy , poaceae , agronomy , botany , tissue culture , genetics , stamen , gene , pollen , in vitro , demography , sociology
Generation of doubled haploid plants is a powerful tool in breeding, as homozygous individuals will be obtained directly from hybrids. However, genotype variability in regeneration efficiency of most European wheat ( Triticum aestivum L.) varieties has limited its use in wheat. This study intended to identify quantitative trait loci ( QTL s) for green plantlet regeneration from wheat microspore cultures. A QTL analysis using DA r T markers was conducted based on a bi‐parental F 3 population, derived from a cross between the varieties Svilena and Jensen, which displayed markedly different capacity for plantlet regeneration. Two QTL s on chromosome 1B and 7B explained 53% of the variation in green plantlet regeneration. Furthermore, a collection of 94 European wheat varieties was genotyped and phenotyped. The microspore response level was low among western and northern European wheat varieties, and the positive QTL s found in the bi‐parental population were rare in the variety collection. Identification of the two QTL s enables introduction of high regeneration efficiency into wheat germplasm. Moreover, our results proved that the efficient regeneration observed for one variety could be crossed into modern winter wheat.