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Identification of a diverse mini‐core panel of I ndian rice germplasm based on genotyping using microsatellite markers
Author(s) -
Tiwari Kapil K.,
Singh Anshuman,
Pattnaik Sasmita,
Sandhu Maninder,
Kaur Sukhdeep,
Jain Sourabh,
Tiwari Sushma,
Mehrotra Shweta,
Anumalla Mahender,
Samal Rashmita,
Bhardwaj Jyoti,
Dubey Neha,
Sahu Vikrant,
Kharshing Gayle A.,
Zeliang Patu K.,
Sreenivasan Kadiri,
Kumar Pankaj,
Parida Swarup K.,
Mithra Sevanthi V. A.,
Rai Vandana,
Tyagi Wricha,
Agrawal Pawan K.,
Rao Atmakuri R.,
Pattanayak Arunava,
Chandel Girish,
Singh Ashok K.,
Bisht Ishwari S.,
Bhat Kangila V.,
Rao Gundimeda J. N.,
Khurana Jitendra P.,
Singh Nagendra K.,
Mohapatra Trilochan
Publication year - 2015
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12252
Subject(s) - germplasm , microsatellite , biology , genotyping , allele , genetic diversity , genotype , locus (genetics) , genetics , amplicon , microbiology and biotechnology , identification (biology) , genetic marker , polymerase chain reaction , agronomy , botany , population , gene , demography , sociology
Identification of a small core germplasm set representing the available genetic diversity is essential for its proper evaluation and subsequent utilization in rice improvement programmes. For constituting a small diverse mini‐core panel of Indian rice germplasm, a representative set of 6912 accessions drawn based on their geographic origin from the whole rice germplasm collection available in the N ational G ene B ank was genotyped using 36 microsatellite markers. Automated fragment analysis of amplicons yielded a total of 435 alleles, with an average 12.4 and range of 3–29 alleles per locus. Polymorphism information content ( PIC ) ranged from 0.08 ( RGNMS 190) to 0.86 ( RM 552) with an average of 0.528. Based on genotyping data, a mini‐core consisting of 98 genotypes was identified. Ninety‐four per cent of the alleles present in the core set were present in the mini‐core. The identified small but diverse panel will be useful for further intensive trait‐specific evaluation and utilization in allele mining.