Premium
Over‐expression of Gh UGP 1 in upland cotton improves fibre quality and reduces fibre sugar content
Author(s) -
Li Bo,
Yang Yang,
Hu WenRan,
Li XiaoDong,
Cao JiaQiang,
Fan Ling
Publication year - 2015
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12247
Subject(s) - sugar , biology , transformation (genetics) , transgene , genetically modified crops , poaceae , food science , cellulose , biochemistry , gene , botany
Gh UGP 1 (cotton uridine diphosphate glucose pyrophosphorylase) is one of the most highly expressed genes and proteins in fast elongation cotton fibres. The objective of this study was to determine whether fibre quality and reduced sugar content could be improved in fibres by over‐expressing Gh UGP 1 in cotton plants under conditions of low autumn temperatures and large diurnal temperature differences. A plant Gh UGP 1 vector was constructed and introduced into the 3‐6‐7 cotton line using agrobacterium‐mediated transformation. Following selection with kanamycin sulphate and polymerase chain reaction ( PCR ) and Southern blotting analyses, three independent homozygous transgenic lines were selected for molecular and chemical study. Compared with wild type, the transcriptional activity of Gh UGP 1 was higher and more long lasting in the transgenic lines. Fibres were longer and stronger, soluble and reduced sugar levels were significantly decreased, and cellulose levels were significantly increased. In addition, plant height also increased by 40.1‐45.1%. These results demonstrate that Gh UGP 1 expression was considerably increased in the transgenic plants, accelerating the conversion of oligosaccharides to polysaccharides in the developing cotton fibre.