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Genome‐wide association studies in elite varieties of German winter barley using single‐marker and haplotype‐based methods
Author(s) -
Gawenda Inka,
Thorwarth Patrick,
Günther Torsten,
Ordon Frank,
Schmid Karl J.
Publication year - 2015
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12237
Subject(s) - biology , genome wide association study , single nucleotide polymorphism , haplotype , quantitative trait locus , genetics , association mapping , genetic association , snp , genetic marker , population , genotype , gene , demography , sociology
Genome‐wide association studies (GWAS) became a widely used method to map qualitative and quantitative traits in plants. We compared existing single‐marker and haplotype‐based methods for GWAS with a focus on barley. Based on German winter barley cultivars, four different single‐marker and haplotype‐based methods were tested for their power to detect significant associations in a large genome with a limited number of markers. We identified significant associations for yield and quality‐related traits using the iSelect array with 3886 mapped single nucleotide polymorphism (SNP) markers in a structured population consisting of 109 genotypes. Genome simulations with different numbers of genotypes, marker densities and marker effects were used to compare different GWAS methods. Results of simulations revealed a higher power in detecting significant associations for haplotype‐ than for single‐marker approaches, but showed a higher false discovery rate for SNP detection, due to lack of correction for population structure. Our simulations revealed that a population size of about 500 individuals is required to detect QTLs explaining a small trait variance (<10%).