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Aluminium tolerance in lentil ( Lens culinaris Medik.) with monogenic inheritance pattern
Author(s) -
Singh Dharmendra,
Dikshit Harsh Kumar,
Kumar Arun
Publication year - 2015
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12227
Subject(s) - backcrossing , biology , hybrid , diallel cross , trait , genetics , botany , gene , horticulture , computer science , programming language
The objectives of this study were to determine genetics of Al tolerance and whether the Al tolerance observed is governed by the same gene. The lines ‘L‐7903’ and ‘L‐4602’ have been developed through breeding programme as Al‐tolerant lines. These lines showed maximum root regrowth and minimum accumulation of Al and callose as compared to sensitive genotypes (‘ BM ‐4’ and ‘L‐4147’). Al tolerance in the parents, F 1 , F 2 and backcross generations was estimated using the regrowth of the primary root after staining and scoring of fluorescent signals. The F 1 hybrids responded similarly to the tolerant parents, indicating dominance of Al tolerance over sensitivity. The segregation ratios obtained for Al tolerance and sensitivity in the F 2 and backcross generations were 3 : 1 and 1 : 1, respectively. Test of allelism confirmed the same gene was conferring Al tolerance in both genotypes (‘L‐7903’ and ‘L‐4602’) as the F 1 was also tolerant and no segregation of tolerant : sensitive was recorded. These results indicated that Al tolerance is a monogenic dominant trait that can be easily transferred to agronomic bases through backcross breeding technique.