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Variations in chlorosis and potential usefulness of alloplasmic B rassica rapa with the cytoplasm of male sterile B rassica juncea
Author(s) -
Chang Caitao,
Sun Deling,
Hondo Kana,
Kakihara Fumika
Publication year - 2014
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12206
Subject(s) - brassica rapa , biology , chlorosis , backcrossing , brassica , botany , hybrid , sterility , cytoplasmic male sterility , horticulture , genetics , gene
To select superior seed parents for vegetable hybrid seed production, we conducted interspecific crosses between male sterile B rassica juncea (2 n = 36, AABB ) and eight inbred lines of B rassica rapa (2 n = 20, AA ). Alloplasmic lines of B . rapa with the cytoplasm of B. juncea were developed from B . juncea × B . rapa hybrids by repeated backcrossing using B. rapa as the recurrent male parent until the BC 3 generation. Seed fertility, male sterility and chlorophyll content were investigated in these plants cultivated under four different temperature conditions (5, 10, 12 and 20°C). At 10°C, the alloplasmic lines of B . rapa with the cytoplasm of B . juncea were male sterile with partly chlorotic leaves. The alloplasmic B. rapa had lower chlorophyll a , chlorophyll b and carotenoid contents than those of the original B . rapa . The leaves recovered from chlorosis when the plants were cultivated at 20°C. An alloplasmic line of B . rapa (A6) is available as a seed parent for vegetable hybrid seed production and contributes seed fertility, slight chlorosis and stable male sterility.