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Mapping of quantitative trait loci conferring resistance to bacterial wilt in tobacco ( Nicotiana tabacum L.)
Author(s) -
Lan Tao,
Zheng Shanfeng,
Yang Liu,
Wu Shengxin,
Wang Bin,
Zhang Shujun,
Tong Zhijun,
Chen Yazhi,
Chen Shunhui,
Duan Yuanlin,
Wu Weiren
Publication year - 2014
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12202
Subject(s) - quantitative trait locus , biology , nicotiana tabacum , genetics , genetic linkage , population , bacterial wilt , curing of tobacco , chromosome , plant disease resistance , marker assisted selection , botany , gene , demography , sociology
Tobacco bacterial wilt (TBW) is one of the most serious tobacco diseases in the world. Studies have shown that tobacco resistance to TBW is quantitatively inherited. This study aimed to map quantitative trait loci (QTL) conferring TBW resistance. An F 2 : 3 population containing 237 lines was developed from a cross between two flue‐cured tobacco cultivars, ‘Yanyan 97’ (YY97; moderately resistant to TBW) and ‘Honghua Dajinyuan’ (HD; highly susceptible to TBW), and a linkage map consisting of 201 simple sequence repeats (SSR) markers and spanning a total length of 2326.7 cM was constructed based on the population. Field experiments were conducted 2011 and 2012, and disease symptoms were investigated three times in each year. The phenotypic data were analysed either separately or jointly for QTL mapping using the software QTLNetwork 2.1. Eight QTL with significant main effects were mapped on chromosomes 2, 6, 12, 17 and 24. A major QTL ( qBWR 17a ) was detected on chromosome 17, which explained up to 30% of the phenotypic variation. The results can facilitate marker‐assisted selection (MAS) in TBW resistance breeding programme.