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Identification of AFLP and SSR markers linked with the male fertility restorer gene of CMS 06J45 in heading C hinese cabbage ( B rassica rapa L. ssp. pekinensis )
Author(s) -
Xu Xiaoyong,
Sun Xilu,
Zhang Yu,
Zhang Lugang,
Fang Zhiyuan
Publication year - 2014
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12200
Subject(s) - biology , amplified fragment length polymorphism , genetics , cytoplasmic male sterility , population , locus (genetics) , primer (cosmetics) , gene , cleaved amplified polymorphic sequence , genetic linkage , genotype , genetic diversity , restriction fragment length polymorphism , sociology , chemistry , organic chemistry , demography
In this study, AFLP and SSR techniques were combined with the bulk segregant analysis ( BSA ) method to map the restorer gene BrRfp using an F 2 ‐segregating population comprising 258 individuals developed by crossing the polima ( pol )‐like cytoplasmic male sterility ( CMS ) line 06J45 and the restorer line 01S325 of heading C hinese cabbage. A survey of 2048 AFLP primer pairs identified 21 polymorphic fragments, approximately half of which exhibited high similarity with the A09 chromosome sequence of B rassica rapa in the B rassica database ( BRAD ). Based on the genome sequence, three specific AFLP fragments linked with BrRfp were successfully converted into sequence‐characterized amplified region ( SCAR ) markers, named SC 1233, SC 2673 and SC 2141. Subsequently, 178 pairs of SSR primers were redesigned for further screening, with five producing polymorphic amplification patterns. Linkage analysis showed that these markers were distributed along both sides of the BrRfp gene, with two markers, SSR 03 and SSR 2528, co‐segregating with the BrRfp locus in the F 2 population. These results may be valuable for marker‐assisted selection and map‐based cloning in heading C hinese cabbage.

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