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Methodology for creating alloplasmic soybean lines by using Glycine tomentella as a maternal parent
Author(s) -
Singh R. J.,
Nelson R. L.
Publication year - 2014
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12196
Subject(s) - biology , backcrossing , botany , subgenus , genetic diversity , glycine , perennial plant , plant morphology , horticulture , gene , genetics , population , demography , amino acid , sociology , genus
Soybean breeders have not exploited the diversity of the 26 wild perennial species of the subgenus Glycine Willd. that are distantly related to soybean [ Glycine max (L.) Merr.] and harbour useful genes. The objective of this study was to develop a methodology for introgressing cytoplasmic and genetic diversity from Glycine tomentella PI 441001 (2 n = 78) into cultivated soybean using ‘Dwight’ (2 n = 40) as the male parent. Immature seeds (19–21 days post‐pollination) were cultured in vitro to produce F 1 plants (2 n = 59). Amphidiploid (2 n = 118) plants, induced by colchicine treatment, were vigorous and produced mature pods and seeds after backcrossing with ‘Dwight’. The BC 1 plants (2 n = 79) produced mature seeds in crosses with ‘Dwight’. Chromosome numbers in BC 2 F 1 plants ranged from 2 n = 41–50. From BC 2 F 2 to BC 3 F 1 , the number of plants in parentheses with 2 n = 40 (275), 2 n = 41 (208), 2 n = 42 (80), 2 n = 43 (27), 2 n = 44 (12) and 2 n = 45 (3) were identified. Fertile lines were grown in the field during 2012 and 2013. This is the first report of the successful development of new alloplasmic soybean lines with cytoplasm from G. tomentella .