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Ectopic expression of Gm PAP 3 enhances salt tolerance in rice by alleviating oxidative damage
Author(s) -
Deng Lixi,
Chen Fen,
Jiang Liping,
Lam HonMing,
Xiao Guoying
Publication year - 2014
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12171
Subject(s) - biology , catalase , malondialdehyde , superoxide dismutase , proline , genetically modified rice , oxidative stress , chlorophyll , horticulture , genetically modified crops , germination , shoot , oryza sativa , botany , transgene , food science , biochemistry , gene , amino acid
Gm PAP 3, a purple acid phosphatase from soybean ( Glycine max ), was previously shown to alleviate salt stress in BY ‐2 cells and Arabidopsis thaliana by reducing oxidative damage. To make use of Gm PAP 3 for crop improvement, we investigated whether the protective function of Gm PAP 3 is persistent in rice. Compared with the untransformed wild type, the transgenic rice plants exhibited enhanced germination rate, longer shoots and roots, and higher survival rate under salt stress, when compared to the untransformed control. In addition, the transgenic plants also showed increased activity of superoxide dismutase ( SOD ) and catalase ( CAT ), proline content, water content and chlorophyll content, but decreased electrolyte leakage and malondialdehyde content in leaves. These results indicate that the expression of Gm PAP 3 could be used to enhance salt tolerance in rice.