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Screening of C hinese cabbage mutants produced by 60 C o γ‐ray mutagenesis of isolated microspore cultures
Author(s) -
Huang Shengnan,
Liu Zhiyong,
Li Danyang,
Yao Runpeng,
Meng Qian,
Feng Hui
Publication year - 2014
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12166
Subject(s) - microspore , mutant , biology , ploidy , mutagenesis , gene , ethyl methanesulfonate , genome , pollen , botany , genetics , stamen
Since the release of the C hinese cabbage genome sequence, increasing interest has focused on the functional analysis of unidentified genes in C hinese cabbage. Mutant analysis forms the basis of functional genomics research. To produce a variety of C hinese cabbage mutants in the same genetic background, buds containing late uninucleate spores from a doubled haploid line of the C hinese cabbage variety ‘ F ukuda 50’ were irradiated with 60 C o γ‐rays at doses of 20, 40 and 60 Gy. Then, the treated microspores were isolated and cultured. A total of 492 putative M 0 mutants were isolated from 1483 regenerated plants. Of these, six M 1 mutants were verified; the mutant frequency was 0.41%. These mutants comprise a mutant library that includes one plant shape mutant, two flower mutants and three male sterile mutants. Pollen viability detection and DNA flow cytometry were used to determine the ploidy of the regenerated plants. Some of the mutants isolated in this study may be useful for C hinese cabbage breeding and functional genomics research.