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Quantitative trait loci mapping of seed colour, hairy leaf, seedling anthocyanin, leaf chlorosis and days to flowering in F 2 population of B rassica rapa L.
Author(s) -
Rahman Mukhlesur,
Mamidi Sujan,
McClean Phillip
Publication year - 2014
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12165
Subject(s) - biology , quantitative trait locus , seedling , chlorosis , population , anthocyanin , botany , gene mapping , genetic linkage , chromosome , genetics , horticulture , gene , demography , sociology
A diversity arrays technology ( DArT ) map was constructed to identify quantitative trait loci ( QTL ) affecting seed colour, hairy leaf, seedling anthocyanin, leaf chlorosis and days to flowering in B rassica rapa using a F 2 population from a cross between two parents with contrasting traits. Two genes with dominant epistatic interaction were responsible for seed colour. One major dominant gene controls the hairy leaf trait. Seedling anthocyanin was controlled by a major single dominant gene. The parents did not exhibit leaf chlorosis; however, 32% F 2 plants showed leaf chlorosis in the population. A distorted segregation was observed for days to flowering in the F 2 population. A linkage map was constructed with 376 DArT markers distributed over 12 linkage groups covering 579.7 cM. The DArT markers were assigned on different chromosomes of B . rapa using B . rapa genome sequences and DArT consensus map of B . napus . Two QTL ( RSC 1‐2 and RSC 12‐56) located on chromosome A8 and chromosome A9 were identified for seed colour, which explained 19.4% and 18.2% of the phenotypic variation, respectively. The seed colour marker located in the ortholog to A rabidopsis thaliana T ransparent T esta2 ( AtTT 2 ). Two QTL RLH 6‐0 and RLH 9‐16 were identified for hairy leaf, which explained 31.6% and 20.7% phenotypic variation, respectively. A single QTL (RSAn‐12‐157) on chromosome A7, which explained 12.8% of phenotypic variation was detected for seedling anthocyanin. The seedling anthocyanin marker is found within the A . thaliana T ransparent T esta12 ( AtTT12 ) ortholog. A QTL (RLC6‐04) for leaf chlorosis was identified, which explained 55.3% of phenotypic variation. QTL for hairy leaf and leaf chlorosis were located 0–4 cM apart on the same chromosome A1. A single QTL ( RDF ‐10‐0) for days to flowering was identified, which explained 21.4% phenotypic variation.