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Conversion of the genic male sterility ( GMS ) system of bell pepper ( Capsicum annuum L.) to cytoplasmic male sterility ( CMS )
Author(s) -
Ou ShangLing,
Chen ShuYun,
Liu SzuChien,
Lo TzuChuan,
Wahyono Andi,
Chen SzYun,
Ku HsinMei
Publication year - 2014
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12145
Subject(s) - pepper , sterility , capsicum annuum , biology , cytoplasmic male sterility , horticulture , hybrid seed , introgression , allele , botany , genetics , gene , hybrid
Non‐pungent bell pepper ( Capsicum annuum L.) lacks the cytoplasmic male sterility ( CMS ) nuclear restorer allele, Rf , and CMS cannot be employed in its F 1 hybrid seed production. To demonstrate that the genic male sterility ( GMS ) system in non‐pungent bell pepper can be converted to the CMS male sterility system, the conversion of GMS to CMS for non‐pungent bell pepper line GC 3 was conducted by introgression of S‐type cytoplasm and the Rf allele from tropical pungent donors. After morphological traits were evaluated, two lines from BC 1 F 1 containing S‐type cytoplasm and four lines from BC 2 F 2 containing Rf allele, phenotypically similar to GC 3, were obtained and could be employed as CMS male sterile lines and restorer lines for non‐pungent bell pepper. Four molecular markers potentially linked to traits of interest were also evaluated in BC 1 F 1 and BC 1 F 2 populations. This is the first time that GMS has been successfully converted to CMS in bell pepper, a significant contribution for bell pepper hybrid seed production.