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Development of NIL s from heterogeneous inbred families for validating the rust resistance QTL in peanut ( A rachis hypogaea L.)
Author(s) -
Yeri Sharanabasappa B.,
Shirasawa Kenta,
Pandey Manish K.,
Gowda M. V. C.,
Sujay Vanhi,
Shriswathi Manda,
Nadaf Hajisaheb L.,
Motagi Babu N.,
Lingaraju S.,
Bhat A. R. S.,
Varshney Rajeev K.,
Krishnaraj P. U.,
Bhat Ramesh S.
Publication year - 2014
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12130
Subject(s) - rust (programming language) , biology , quantitative trait locus , inbred strain , allele , plant disease resistance , genetics , botany , horticulture , gene , computer science , programming language
Heterogeneous inbred families segregating for rust resistance were identified from the two crosses involving susceptible ( TAG 24 and TG 26) and resistant ( GPBD 4) varieties of peanut. Rust‐resistant (less than score 5) and rust‐susceptible (more than score 5) plants were identified in each HIF and evaluated under rust epiphytotic conditions. The set of plants belonging to the same HIF , but differing significantly in rust resistance, not in other morphological and productivity traits, was regarded as near‐isogenic lines (NILs). Largely, rust‐resistant NIL s had GPBD 4‐type allele, and susceptible NIL s carried either TAG 24 or TG 26‐type allele at the three SSR loci ( IPAHM 103, GM 1536 and GM 2301) linked to a major genomic region governing rust resistance. Comparison of the remaining genomic regions between the NIL s originating from each of the HIF s using transposon markers indicated a considerably high similarity of 86.4% and 83.1% in TAG 24 × GPPBD 4 and TG 26 × GPBD 4, respectively. These NIL s are useful for fine mapping and expression analysis of rust resistance.