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The first genetic linkage map of crape myrtle ( L agerstroemia ) based on amplification fragment length polymorphisms and simple sequence repeats markers
Author(s) -
He Dan,
Liu Yang,
Cai Ming,
Pan Huitang,
Zhang Qixiang
Publication year - 2014
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12100
Subject(s) - amplified fragment length polymorphism , biology , genetics , genetic linkage , microsatellite , genotyping , linkage (software) , gene mapping , genetic marker , genetic diversity , chromosome , genotype , population , allele , gene , demography , sociology
Lagerstroemia (crape myrtle) are famous ornamental plants with large pyramidal racemes, long flower duration and diverse colours. Genetic maps provide an important genomic resource of basic and applied significance. A genetic linkage map was developed by genotyping 192 F 1 progeny from a cross between L . caudata (female) and L . indica (‘ X iang X ue Y un’) (male) with a combination of amplification fragment length polymorphisms ( AFLP ) and simple sequence repeats ( SSR ) markers in a double pseudo‐testcross mapping strategy. A total of 330 polymorphic loci consisting of 284 AFLP s and 46 SSR s showing M endelian segregation were generated from 383 AFLP primer combinations and 150 SSR primers. The data were analysed using JoinMap 4.0 (evaluation version) to construct the linkage map. The map consisted of 20 linkage groups of 173 loci (160 AFLP s and 13 SSR s) covering 1162.1 cM with a mean distance of 10.69 cM between adjacent markers. The 20 linkage groups contained 2–49 loci and ranged in length from 7.38 to 163.57 cM. This map will serve as a framework for mapping QTLs and provide reference information for future molecular breeding work.