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Development of molecular markers from Indian genotypes of two Gossypium L. species
Author(s) -
Srivastava Anukool,
Jena Satya Narayan,
Ranjan Alok,
Kavita Palacharla,
Asif Mehar H.,
Bag Sumit K.,
Shukla Ravi Prakash,
Yadav Hemant K.,
Sawant Samir V.
Publication year - 2013
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12087
Subject(s) - indel , biology , transversion , single nucleotide polymorphism , genotype , gossypium hirsutum , genetics , genetic marker , molecular marker , molecular breeding , transcriptome , gene , botany , mutation , gene expression
Two genotypes of Gossypium hirsutum and four genotypes of G. herbaceum have been used to develop large‐scale molecular markers, especially SSR s and SNP s for their use in various molecular breeding programmes of cotton. A total of 1440 expressed SSR s and 2608 SNP s were identified from transcriptome of two genotypes of G. hirsutum . In addition, 10 971 SNP s were also developed from transcriptome of G. herbaceum, of which 4059 SNP s were transition and 6912 were transversion and there were 238 indels. This study added a total of 334 novel SSR s and 1847 novel SNP s in G. hirsutum and 10 947 novel SNP s in G. herbaceum to the public domain. We also validated a subset of 25 novel SSR s and 12 SNP s with five genotypes of G. hirsutum .