Development of molecular markers from Indian genotypes of two  Gossypium  L. species | Zendy

Srivastava Anukool | Zendy; Jena Satya Narayan | Zendy; Ranjan Alok | Zendy; Kavita Palacharla | Zendy; Asif Mehar H. | Zendy; Bag Sumit K. | Zendy; Shukla Ravi Prakash | Zendy; Yadav Hemant K. | Zendy; Sawant Samir V. | Zendy

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Development of molecular markers from Indian genotypes of two Gossypium L. species

Author(s) -

Srivastava Anukool,

Jena Satya Narayan,

Ranjan Alok,

Kavita Palacharla,

Asif Mehar H.,

Bag Sumit K.,

Shukla Ravi Prakash,

Yadav Hemant K.,

Sawant Samir V.

Publication year - 2013

Publication title -

plant breeding

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.583

H-Index - 71

eISSN - 1439-0523

pISSN - 0179-9541

DOI - 10.1111/pbr.12087

Subject(s) - indel , biology , transversion , single nucleotide polymorphism , genotype , gossypium hirsutum , genetics , genetic marker , molecular marker , molecular breeding , transcriptome , gene , botany , mutation , gene expression

Two genotypes of Gossypium hirsutum and four genotypes of G. herbaceum have been used to develop large‐scale molecular markers, especially SSR s and SNP s for their use in various molecular breeding programmes of cotton. A total of 1440 expressed SSR s and 2608 SNP s were identified from transcriptome of two genotypes of G. hirsutum . In addition, 10 971 SNP s were also developed from transcriptome of G. herbaceum, of which 4059 SNP s were transition and 6912 were transversion and there were 238 indels. This study added a total of 334 novel SSR s and 1847 novel SNP s in G. hirsutum and 10 947 novel SNP s in G. herbaceum to the public domain. We also validated a subset of 25 novel SSR s and 12 SNP s with five genotypes of G. hirsutum .

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