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Novel molecular marker associated with T m2 a gene conferring resistance to tomato mosaic virus in tomato
Author(s) -
Panthee Dilip R.,
Brown Allan F.,
Yousef Gad G.,
Ibrahem Ragy,
Anderson Candice
Publication year - 2013
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12076
Subject(s) - biology , gene , genetics , genotype , locus (genetics) , restriction enzyme , cleaved amplified polymorphic sequence , molecular marker , genetic marker , marker assisted selection , phenotype , virus , r gene , plant disease resistance , microbiology and biotechnology , restriction fragment length polymorphism
Tomato mosaic virus ( T o MV ) is an important T obamovirus that causes significant crop losses. Resistance to the T o MV is conferred by the genes T m1 , T m2 and T m2 a . Among these three genes, T m2 a confers resistance to most strains of the T o MV . Screening of genetic lines under field conditions based on phenotype is time‐consuming and challenging due to concerns associated with stability of the virus and its potential transmission to other plants. Tightly linked molecular markers associated with resistance genes can improve selection efficiency and avoid these problems. This study developed a PCR ‐based marker based on restriction site differences from T m2 a locus‐specific sequences, which was found to be useful in identifying the resistant and susceptible genotypes and was consistent with phenotypic data. The marker is a codominant cleaved amplified polymorphic sequence ( CAPS ) marker producing 270‐ and 600‐bp DNA fragments from resistant genotypes and an 870‐bp fragment from susceptible genotypes when digested with Hae III restriction enzyme. This novel marker can be useful for tomato breeders to screen progeny from segregating populations for T o MV resistance.