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Micro RNA ‐based molecular markers: a novel PCR ‐based genotyping technique in Brassica species
Author(s) -
Fu Donghui,
Ma Bi,
Mason Annaliese S.,
Xiao Meili,
Wei Lijuan,
An Zeshan
Publication year - 2013
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12069
Subject(s) - biology , genotyping , rna , genetics , primer (cosmetics) , brassica , transferability , molecular marker , germplasm , genetic marker , dna , polymerase chain reaction , computational biology , genetic diversity , gene , microbiology and biotechnology , genotype , botany , population , chemistry , statistics , mathematics , organic chemistry , logit , demography , sociology
DNA ‐based molecular markers play a significant role in gene mapping, genetic diversity analysis, germplasm evaluation and molecular marker‐assisted selection. A combination of desirable marker characteristics such as abundant polymorphism, good stability, ease of production and high efficiency is difficult to achieve when utilizing traditional molecular marker systems. Micro RNA s are a type of endogenous non‐coding RNA s prevalent in the genomes of many organisms. The high conservation of mi RNA and pre‐mi RNA sequences provides an opportunity to develop a novel molecular marker type. We downloaded 82 mi RNA sequences from the Brassica mi RB ase database and designed 46 single mi RNA ‐based primers which could be randomly combined to generate primer pairs. A proportion of these primer pairs were validated for transferability and polymorphism using DNA from 16 varieties of six Brassica species. The percentage of polymorphic primer pairs were 28.1%, and the average polymorphism information content ( PIC ) value for the 34 primer pairs was 0.43. Good transferability was verified across species. These results indicate that mi RNA ‐based markers provide a novel genotyping technique with low costs, high efficiency, stability and good transferability.