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Interspecific hybridization in G ossypium L.: characterization of progenies with different ploidy‐confirmed multigenomic backgrounds
Author(s) -
Newaskar Ganesh S.,
Chimote Vivek P.,
Mehetre Subhash S.,
Jadhav Ashok S.
Publication year - 2013
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12031
Subject(s) - biology , ploidy , introgression , microsatellite , gossypium , botany , genetics , interspecific competition , hybrid , allele , gene
Cytological and molecular investigations were undertaken for parent and progeny derived from a trispecific line [2( G ossypium arboreum × G . anomalum ) × G . hirsutum var. BWR ], which was crossed with G . hirsutum var. JLH 168. Cytomorphological analysis of the F 1 ( G . arboreum × G . anomalum ), its amphidiploid and progeny from trispecies hybrid showed distorted ploidy segregation with monovalents to hexavalents and high intergenomic (small A 2 and large B 1 ) allosynthetic chromosome pairing. Microsatellite analysis identified three fragments associated with G . arboreum and G . anomalum and six fragments associated with G . hirsutum in derivates of the trispecies line × G . hirsutum var. JLH 168. Inter‐Retrotransposon Amplified Polymorphism ( IRAP ) analysis revealed fragments of G . arboreum and G . anomalum , only in F 1 and amphidiploid. Chromosomal association and microsatellite analysis of three progeny genotypes (i.e. haploid, hexaploid and tetraploid no. 1) confirmed that they share multigenomic background from the three cotton species (A 2 , A h D h and B 1 genome). The interspecific hybrid cotton genotypes studied are likely to be useful for the introgression of genes from diploid species to commercial upland cultivars.