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Marker‐assisted breeding of Pi‐1 and Piz‐5 genes imparting resistance to rice blast in PRR 78, restorer line of P usa RH ‐10 B asmati rice hybrid
Author(s) -
Gouda Patil K.,
Saikumar Surapaneni,
Varma Chejerla M. K.,
Nagesh Kancharla,
Thippeswamy Sanka,
Shenoy Vinay,
Ramesha Mugalodim S.,
Shashidhar Halagappa E.
Publication year - 2013
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12017
Subject(s) - backcrossing , introgression , biology , hybrid , magnaporthe grisea , plant disease resistance , gene , pusa , background selection , cultivar , marker assisted selection , blast disease , genetics , horticulture , genotype , oryza sativa
Rice blast, caused by fungus M agnaporthe grisea, is a serious disease causing considerable economic damage worldwide. Best way to overcome disease is to breed for disease‐resistant cultivars/parental lines of hybrids. Pusa RH 10, first aromatic, fine‐grain rice hybrid released and cultivated extensively in India. Hybrid and its parental lines, P usa 6 A and PRR 78, are highly susceptible to blast. CO 39 pyramid carrying two dominant, broad‐spectrum blast‐resistance genes, viz. Pi‐1 and Piz‐5 , used as a donor parent to introgress these genes into PRR 78 using marker‐assisted backcrossing ( MABC ). Microsatellite markers RM 5926 and AP 5659‐5 tightly linked to Pi‐1 and Piz‐5 genes, respectively, were used for foreground selection to derive introgression lines. Further, these lines were evaluated for agronomic performance, disease reaction and cooking quality traits along with PRR 78. Most of the improved lines were on par with PRR 78 for all traits evaluated except gelatinization temperature. Recurrent parent genome percentage ( RPG ) study also revealed similarity of these lines with PRR 78. Hybrids derived using improved PRR 78 lines were superior over P usa RH 10 in terms of yield.