Open AccessHigh‐throughput sequencing revealed low‐efficacy genome editing using Cas9 RNPs electroporation and single‐celled microinjection provided an alternative to deliver CRISPR reagents into Euglena gracilis
Author(s) -
Chen Zhenfan,
Zhu Jiayi,
Chen Zixi,
Du Ming,
Yao Rao,
Fu Wen,
Lei Anping,
Wang Jiangxin
Publication year - 2022
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.13915
Subject(s) - euglena gracilis , biology , crispr , genome editing , cas9 , genome , guide rna , subgenomic mrna , electroporation , genetics , computational biology , gene , chloroplast