Open AccessNatural variation of HTH5 from wild rice, Oryza rufipogon Griff., is involved in conferring high‐temperature tolerance at the heading stage
Author(s) -
Cao Zhibin,
Tang Huiwu,
Cai Yaohui,
Zeng Bohong,
Zhao Jialiang,
Tang Xiuying,
Lu Ming,
Wang Huimin,
Zhu Xuejing,
Wu Xiaofeng,
Yuan Linfeng,
Wan Jianlin
Publication year - 2022
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.13835
Subject(s) - biology , oryza rufipogon , oryza sativa , quantitative trait locus , oryza , abiotic component , abiotic stress , cultivar , botany , gene , genetically modified rice , genetics , horticulture , transgene , genetically modified crops , ecology
Summary Global warming is a major abiotic stress factor, which limit rice production. Exploiting the genetic basis of the natural variation in heat resistance at different reproductive stages among diverse exotic Oryza germplasms can help breeding heat‐resistant rice cultivars. Here, we identified a stable quantitative trait locus (QTL) for heat tolerance at the heading stage on chromosome 5 ( qHTH5 ) in O . rufipogon Griff. The corresponding gene, HTH5 , pertains to the pyridoxal phosphate‐binding protein PLPBP (formerly called PROSC) family, which is predicted to encode pyridoxal phosphate homeostasis protein (PLPHP) localized to the mitochondrion. Overexpression of HTH5 increased the seed‐setting rate of rice plants under heat stress at the heading stage, whereas suppression of HTH5 resulted in greater susceptibility to heat stress. Further investigation indicated that HTH5 reduces reactive oxygen species accumulation at high temperatures by increasing the heat‐induced pyridoxal 5'‐phosphate (PLP) content. Moreover, we found that two SNPs located in the HTH5 promoter region are involved with its expression level and associated with heat tolerance diversity. These findings suggest that the novel gene HTH5 might have great potential value for heightening rice tolerance to heat stress to the on‐going threat of global warming.