Open AccessDevelopment of an efficient marker‐free soybean transformation method using the novel bacterium Ochrobactrum haywardense H1
Author(s) -
Cho HyeonJe,
Moy York,
Rudnick Nathan A.,
Klein Theodore M.,
Yin Jiaming,
Bolar Joy,
Hendrick Carol,
Beatty Mary,
Castañeda Leandro,
Kinney Anthony J.,
Jones Todd J.,
Chilcoat N. Doane
Publication year - 2022
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.13777
Subject(s) - biology , transformation (genetics) , selectable marker , agrobacterium , transformation efficiency , agrobacterium tumefaciens , plasmid , genetically modified crops , transgene , auxotrophy , genetics , gene , mutant
Summary We have discovered a novel bacterium, Ochrobactrum haywardense H1 ( Oh H1), which is capable of efficient plant transformation. Ochrobactrum is a new host for Agrobacterium ‐derived vir and T‐DNA‐mediated transformation. Oh H1 is a unique, non‐phytopathogenic species, categorized as a BSL‐1 organism. We engineered Oh H1 with repurposed Agrobacterium virulence machinery and demonstrated Oh H1 can transform numerous dicot species and at least one monocot, sorghum. We generated a cysteine auxotrophic Oh H1‐8 strain containing a binary vector system. Oh H1‐8 produced transgenic soybean plants with an efficiency 1.6 times that of Agrobacterium strain AGL1 and 2.9 times that of LBA4404Thy‐. Oh H1‐8 successfully transformed several elite Corteva soybean varieties with T0 transformation frequency up to 35%. In addition to higher transformation efficiencies, Oh H1‐8 generated high‐quality, transgenic events with single‐copy, plasmid backbone‐free insertion at frequencies higher than AGL1. The SpcN selectable marker gene is excised using a heat shock‐inducible excision system resulting in marker‐free transgenic events. Approximately, 24.5% of the regenerated plants contained only a single copy of the transgene and contained no vector backbone. There were no statistically significant differences in yield comparing T3 null‐segregant lines to wild‐type controls. We have demonstrated that Oh H1‐8, combined with spectinomycin selection, is an efficient, rapid, marker‐free and yield‐neutral transformation system for elite soybean.