Open AccessCRISPR/Cas‐based precision genome editing via microhomology‐mediated end joining
Author(s) -
Vu Tien,
Doan Duong,
Kim Jihae,
Sung Yeon Woo,
Tran Mil,
Song Young Jong,
Das Swati,
Kim JaeYean
Publication year - 2021
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.13490
Subject(s) - genome editing , crispr , biology , transcription activator like effector nuclease , genome , computational biology , genome engineering , genetics , rna editing , zinc finger nuclease , cas9 , gene , mutagenesis , mutation , intron
Summary Gene editing and/or allele introgression with absolute precision and control appear to be the ultimate goals of genetic engineering. Precision genome editing in plants has been developed through various approaches, including oligonucleotide‐directed mutagenesis (ODM), base editing, prime editing and especially homologous recombination (HR)‐based gene targeting. With the advent of CRISPR/Cas for the targeted generation of DNA breaks (single‐stranded breaks (SSBs) or double‐stranded breaks (DSBs)), a substantial advancement in HR‐mediated precise editing frequencies has been achieved. Nonetheless, further research needs to be performed for commercially viable applications of precise genome editing; hence, an alternative innovative method for genome editing may be required. Within this scope, we summarize recent progress regarding precision genome editing mediated by microhomology‐mediated end joining (MMEJ) and discuss their potential applications in crop improvement.