Open AccessTranscriptional landscape of pathogen‐responsive lnc RNA s in rice unveils the role of ALEX 1 in jasmonate pathway and disease resistance
Author(s) -
Yu Yang,
Zhou YanFei,
Feng YanZhao,
He Huang,
Lian JianPing,
Yang YuWei,
Lei MengQi,
Zhang YuChan,
Chen YueQin
Publication year - 2020
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.13234
Subject(s) - biology , rna , jasmonate , gene , non coding rna , gene expression , plant disease resistance , rna silencing , genetics , regulation of gene expression , microbiology and biotechnology , rna interference , arabidopsis , mutant
Summary Plant defence is multilayered and is essential for surviving in a changing environment. The discovery of long noncoding RNA s (lnc RNA s) has dramatically extended our understanding of post‐transcriptional gene regulation in diverse biological processes. However, the expression profile and function of lnc RNA s in disease resistance are still largely unknown, especially in monocots. Here, we performed strand‐specific RNA sequencing of rice leaves infected by Xanthomonas oryzae pv. Oryzae ( Xoo ) in different time courses and systematically identified 567 disease‐responsive rice lnc RNA s. Target analyses of these lnc RNA s showed that jasmonate ( JA ) pathway was significantly enriched. To reveal the interaction between lnc RNA s and JA ‐related genes, we studied the coexpression of them and found 39 JA ‐related protein‐coding genes to be interplayed with 73 lnc RNA s, highlighting the potential modulation of lnc RNA s in JA pathway. We subsequently identified an lnc RNA , ALEX 1, whose expression is highly induced by Xoo infection. A T‐ DNA insertion line constructed using enhancer trap system showed a higher expression of ALEX 1 and exerted a significant resistance to rice bacterial blight. Functional study revealed that JA signalling is activated and the endogenous content of JA and JA ‐Ile is increased. Overexpressing ALEX 1 in rice further confirmed the activation of JA pathway and resistance to bacterial blight. Our findings reveal the expression of pathogen‐responsive lnc RNA s in rice and provide novel insights into the connection between lnc RNA s and JA pathway in the regulation of plant disease resistance.