Open AccessMutations in MIR 396e and MIR 396f increase grain size and modulate shoot architecture in rice
Author(s) -
Miao Chunbo,
Wang Dong,
He Reqing,
Liu Shenkui,
Zhu JianKang
Publication year - 2020
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.13214
Subject(s) - biology , plant stem , gibberellin , mutant , gene , elongation , shoot , crop , gibberellic acid , grain yield , botany , agronomy , horticulture , microbiology and biotechnology , genetics , germination , materials science , ultimate tensile strength , metallurgy
Summary Grain size and plant architecture are critical factors determining crop productivity. Here, we performed gene editing of the MIR 396 gene family in rice and found that MIR 396e and MIR 396f are two important regulators of grain size and plant architecture. mir396ef mutations can increase grain yield by increasing grain size. In addition, mir396ef mutations resulted in an altered plant architecture, with lengthened leaves but shortened internodes, especially the uppermost internode. Our research suggests that mir396ef mutations promote leaf elongation by increasing the level of a gibberellin ( GA ) precursor, mevalonic acid, which subsequently promotes GA biosynthesis. However, internode elongation in mir396ef mutants appears to be suppressed via reduced CYP 96B4 expression but not via the GA pathway. This research provides candidate gene‐editing targets to breed elite rice varieties.