Open AccessHighly efficient transgene‐free targeted mutagenesis and single‐stranded oligodeoxynucleotide‐mediated precise knock‐in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins
Author(s) -
Nomura Toshihisa,
Inoue Komaki,
UeharaYamaguchi Yukiko,
Yamada Koji,
Iwata Osamu,
Suzuki Kengo,
Mochida Keiichi
Publication year - 2019
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.13174
Subject(s) - biology , euglena gracilis , cas9 , transgene , mutagenesis , crispr , genome editing , genetics , ribonucleoprotein , computational biology , mutation , gene , chloroplast , rna