Open AccessOptimizing base editors for improved efficiency and expanded editing scope in rice
Author(s) -
Wang Mugui,
Wang Zhidan,
Mao Yanfei,
Lu Yuming,
Yang Ruifang,
Tao Xiaoping,
Zhu JianKang
Publication year - 2019
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.13124
Subject(s) - cytidine deaminase , biology , genome editing , cytidine , cas9 , crispr , computational biology , gene , base (topology) , genetics , biochemistry , enzyme , mathematical analysis , mathematics
Base editors, presently including cytidine base editors (CBEs) and adenine base editors (ABEs), enable precise base alterations in the genome without inducing DNA double-stranded breaks (DSBs). Base editors are valuable tools for precision plant molecular breeding since many agronomic traits are controlled by variations in one or few DNA bases. The early developed CBE and ABE systems, consisting of the rat cytidine deaminase APOBEC1 (rAPOBEC1) or activation-induced cytidine deaminase (AID) PmCDA1, and the evolved tRNA adenine deaminase TadA, respectively, have been applied to many plant species. This article is protected by copyright. All rights reserved.