Open AccessTracking disease resistance deployment in potato breeding by enrichment sequencing
Author(s) -
Armstrong Miles R.,
Vossen Jack,
Lim Tze Yin,
Hutten Ronald C. B.,
Xu Jianfei,
Strachan Shona M.,
Harrower Brian,
Champouret Nicolas,
Gilroy Eleanor M.,
Hein Ingo
Publication year - 2019
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12997
Subject(s) - phytophthora infestans , biology , plant disease resistance , gene , blight , genetics , genome , resistance (ecology) , microbiology and biotechnology , computational biology , agronomy
Summary Following the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing ( dR enSeq) enables the high‐confidence identification and complete sequence validation of known functional resistance genes in crops. As demonstrated for tetraploid potato varieties, the methodology is more robust and cost‐effective in monitoring resistances than whole‐genome sequencing and can be used to appraise (trans) gene integrity efficiently. All currently known NB ‐ LRR s effective against viruses, nematodes and the late blight pathogen Phytophthora infestans can be tracked with dR enSeq in potato and hitherto unknown polymorphisms have been identified. The methodology provides a means to improve the speed and efficiency of future disease resistance breeding in crops by directing parental and progeny selection towards effective combinations of resistance genes.