Open AccessQTL sequencing strategy to map genomic regions associated with resistance to ascochyta blight in chickpea
Author(s) -
Deokar Amit,
Sagi Mandeep,
Daba Ketema,
Tar'an Bunyamin
Publication year - 2019
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12964
Subject(s) - ascochyta , biology , quantitative trait locus , blight , resistance (ecology) , genetics , gene , agronomy
Summary Whole‐genome sequencing‐based bulked segregant analysis ( BSA ) for mapping quantitative trait loci ( QTL ) provides an efficient alternative approach to conventional QTL analysis as it significantly reduces the scale and cost of analysis with comparable power to QTL detection using full mapping population. We tested the application of next‐generation sequencing ( NGS )‐based BSA approach for mapping QTL s for ascochyta blight resistance in chickpea using two recombinant inbred line populations CPR ‐01 and CPR ‐02. Eleven QTL s in CPR ‐01 and six QTL s in CPR ‐02 populations were mapped on chromosomes Ca1, Ca2, Ca4, Ca6 and Ca7. The QTL s identified in CPR ‐01 using conventional biparental mapping approach were used to compare the efficiency of NGS ‐based BSA in detecting QTL s for ascochyta blight resistance. The QTL s on chromosomes Ca1, Ca4, Ca6 and Ca7 overlapped with the QTL s previously detected in CPR ‐01 using conventional QTL mapping method. The QTL s on chromosome Ca4 were detected in both populations and overlapped with the previously reported QTL s indicating conserved region for ascochyta blight resistance across different chickpea genotypes. Six candidate genes in the QTL regions identified using NGS ‐based BSA on chromosomes Ca2 and Ca4 were validated for their association with ascochyta blight resistance in the CPR ‐02 population. This study demonstrated the efficiency of NGS ‐based BSA as a rapid and cost‐effective method to identify QTL s associated with ascochyta blight in chickpea.