Open AccessChromosome‐based survey sequencing reveals the genome organization of wild wheat progenitor Triticum dicoccoides
Author(s) -
Akpinar Bala Ani,
Biyiklioglu Sezgi,
Alptekin Burcu,
Havránková Miroslava,
Vrána Jan,
Doležel Jaroslav,
Distelfeld Assaf,
Hernandez Pilar,
Budak Hikmet
Publication year - 2018
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12940
Subject(s) - biology , synteny , genetics , genome , gene , chromosome , dna sequencing
Summary Wild emmer wheat ( Triticum turgidum ssp. dicoccoides ) is the progenitor of wheat. We performed chromosome‐based survey sequencing of the 14 chromosomes, examining repetitive sequences, protein‐coding genes, mi RNA /target pairs and tRNA genes, as well as syntenic relationships with related grasses. We found considerable differences in the content and distribution of repetitive sequences between the A and B subgenomes. The gene contents of individual chromosomes varied widely, not necessarily correlating with chromosome size. We catalogued candidate agronomically important loci, along with new alleles and flanking sequences that can be used to design exome sequencing. Syntenic relationships and virtual gene orders revealed several small‐scale evolutionary rearrangements, in addition to providing evidence for the 4 AL ‐5 AL ‐7 BS translocation in wild emmer wheat. Chromosome‐based sequence assemblies contained five novel mi RNA families, among 59 families putatively encoded in the entire genome which provide insight into the domestication of wheat and an overview of the genome content and organization.