Open AccessZm STK 1 and Zm STK 2 , encoding receptor‐like cytoplasmic kinase, are involved in maize pollen development with additive effect
Author(s) -
Fan Mingxia,
Zhang Chunyu,
Shi Lei,
Liu Chen,
Ma Wenjuan,
Chen Meiming,
Liu Kuichen,
Cai Fengchun,
Wang Guohong,
Wei Zhengyi,
Jiang Min,
Liu Zaochang,
Javeed Ansar,
Lin Feng
Publication year - 2018
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12880
Subject(s) - biology , pollen , kinase , signal transduction , biochemistry , microbiology and biotechnology , pollen tube , botany , pollination
Summary Pollen germination and pollen tube growth are important physiological processes of sexual reproduction of plants and also are involved in signal transduction. Our previous study reveals that Zm STK 1 and Zm STK 2 are two receptor‐like cytoplasmic kinases ( RLCK ) homologs in Zea mays as members of receptor‐like protein kinase ( RLK ) subfamily, sharing 86% identity at the amino acid level. Here, we report that Zm STK 1 and Zm STK 2 , expressed at late stages of pollen development, regulate maize pollen development with additive effect. Zm STK 1 or Zm STK 2 mutation exhibited severe pollen transmission deficiency, which thus influenced pollen fertility. Moreover, the kinase domains of Zm STK s were cross‐interacted with C‐terminus of enolases detected by co‐immunoprecipitation (Co‐ IP ) and yeast two‐hybrid system (Y2H), respectively. Further, the detective Zm STK 1 or Zm STK 2 was associated with decreased activity of enolases and also reduced downstream metabolite contents, which enolases are involved in glycolytic pathway, such as phosphoenolpyruvate ( PEP ), pyruvate, ADP / ATP , starch, glucose, sucrose and fructose. This study reveals that Zm STK 1 and Zm STK 2 regulate maize pollen development and indirectly participate in glycolytic pathway.