Open AccessChrysanthemum Cm HSFA 4 gene positively regulates salt stress tolerance in transgenic chrysanthemum
Author(s) -
Li Fei,
Zhang Huanru,
Zhao Husheng,
Gao Tianwei,
Song Aiping,
Jiang Jiafu,
Chen Fadi,
Chen Sumei
Publication year - 2018
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12871
Subject(s) - salinity , apx , biology , transcription factor , activator (genetics) , transgene , microbiology and biotechnology , heat shock protein , oxidative stress , heat shock factor , genetically modified crops , gene expression , gene , botany , biochemistry , hsp70 , catalase , ecology
Summary Salinity‐induced Na + toxicity and oxidative stress hamper plant growth. Here, we showed that expression of the chrysanthemum Cm HSFA 4 , a homologue of the heat‐shock factor At HSFA 4a , is inducible by salt and localizes to the nucleus. It is a transcription activator binding with HSE . Chrysanthemum overexpressing Cm HSFA 4 displayed enhanced salinity tolerance by limiting Na + accumulation while maintaining K + concentration, which is consistent with the up‐regulation of ion transporters Cm SOS 1 and Cm HKT 2 . Additionally, the transgenic plants reduced H 2 O 2 and O 2 ∙− accumulation under salinity, which could be due to up‐regulation of ROS scavenger activities such as SOD , APX and CAT as well as Cm HSP 70 , Cm HSP 90 . Together, these results suggest that Cm HSFA 4 conferred salinity tolerance in chrysanthemum as a consequence of Na + /K + ion and ROS homeostasis.