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Expression and assembly of largest foreign protein in chloroplasts: oral delivery of human FVIII made in lettuce chloroplasts robustly suppresses inhibitor formation in haemophilia A mice
Author(s) -
Kwon KwangChul,
Sherman Alexandra,
Chang WanJung,
Kamesh Aditya,
Biswas Moanaro,
Herzog Roland W.,
Daniell Henry
Publication year - 2018
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12859
Subject(s) - haemophilia a , biology , population , microbiology and biotechnology , recombinant dna , chloroplast , immunology , biochemistry , haemophilia , gene , medicine , genetics , environmental health
Summary Inhibitor formation is a serious complication of factor VIII ( FVIII ) replacement therapy for the X‐linked bleeding disorder haemophilia A and occurs in 20%–30% of patients. No prophylactic tolerance protocol currently exists. Although we reported oral tolerance induction using FVIII domains expressed in tobacco chloroplasts, significant challenges in clinical advancement include expression of the full‐length CTB ‐ FVIII sequence to cover the entire patient population, regardless of individual CD 4 + T‐cell epitope responses. Codon optimization of FVIII heavy chain ( HC ) and light chain ( LC ) increased expression 15‐ to 42‐fold higher than the native human genes. Homoplasmic lettuce lines expressed CTB fusion proteins of FVIII ‐ HC (99.3 kDa), LC (91.8 kDa), C2 (31 kDa) or single chain ( SC , 178.2 kDa) up to 3622, 263, 3321 and 852 μg/g in lyophilized plant cells, when grown in a cGMP hydroponic facility (Fraunhofer). CTB ‐ FVIII ‐ SC is the largest foreign protein expressed in chloroplasts; despite a large pentamer size (891 kDa), assembly, folding and disulphide bonds were maintained upon lyophilization and long‐term storage as revealed by GM 1‐ganglioside receptor binding assays. Repeated oral gavages (twice/week for 2 months) of CTB ‐ FVIII ‐ HC / CTB ‐ FVIII ‐ LC reduced inhibitor titres ~10‐fold (average 44 BU/mL to 4.7 BU/mL) in haemophilia A mice. Most importantly, increase in the frequency of circulating LAP ‐expressing CD 4 + CD 25 + FoxP3 + Treg in tolerized mice could be used as an important cellular biomarker in human clinical trials for plant‐based oral tolerance induction. In conclusion, this study reports the first clinical candidate for oral tolerance induction that is urgently needed to protect haemophilia A patients receiving FVIII injections.

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