Open AccessTranscriptomic repertoires depict the initiation of lint and fuzz fibres in cotton ( Gossypium hirsutum L.)
Author(s) -
Hu Haiyan,
Wang Maojun,
Ding Yuanhao,
Zhu Sitao,
Zhao Guannan,
Tu Lili,
Zhang Xianlong
Publication year - 2018
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12844
Subject(s) - lint , biology , rna , transcriptome , gossypium , rna silencing , mutant , gene , gene silencing , genetics , rna interference , gene expression , agronomy
Summary Cotton fibre is an important natural fibre for the textile industry. The number of fibre initials determines the lint percentage, which is an important factor for cotton fibre yield. Although fibre development has been described by transcriptomic analysis, the mechanism by which the long noncoding RNA manipulates the initiation of lint and fuzz fibres remains unknown. In this study, three lines with different lint percentages were developed by crossing Xu142 with its fibreless mutant Xu142 fl . We collected the epidermal cells from the ovules with attached fibres at 0 and 5 days post anthesis ( DPA ) from Xu142, the fibreless mutant Xu142 fl and the three lint percent diversified lines for deep transcriptome sequencing. A total of 2641 novel genes, 35 802 long noncoding RNA s (lnc RNA s) and 2262 circular RNA s (circ RNA s) were identified, of which 645 lnc RNA s were preferentially expressed in the fibreless mutant Xu142 fl and 651 lnc RNA s were preferentially expressed in the fibre‐attached lines. We demonstrated the functional roles of the three lnc RNA s in fibre development via a virus‐induced gene silencing ( VIGS ) system. Our results showed that silencing XLOC _545639 and XLOC _039050 in Xu142 fl increased the number of fibre initials on the ovules, but silencing XLOC _079089 in Xu142 resulted in a short fibre phenotype. This study established the transcriptomic repertoires in cotton fibre initiation and provided evidence for the potential functions of lnc RNA s in fibre development.