Open AccessOs ASR 2 regulates the expression of a defence‐related gene, Os2H16 , by targeting the GT ‐1 cis ‐element
Author(s) -
Li Ning,
Wei Shutong,
Chen Jing,
Yang Fangfang,
Kong Lingguang,
Chen Cuixia,
Ding Xinhua,
Chu Zhaohui
Publication year - 2018
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12827
Subject(s) - biology , gene , xanthomonas oryzae , abscisic acid , abiotic stress , promoter , gene expression , crosstalk , genetics , oryza sativa , plant disease resistance , osmotic shock , trans acting , microbiology and biotechnology , physics , optics , mutant
Summary The GT ‐1 cis ‐element widely exists in many plant gene promoters. However, the molecular mechanism that underlies the response of the GT ‐1 cis ‐element to abiotic and biotic stresses remains elusive in rice. We previously isolated a rice short‐chain peptide‐encoding gene, Os2H16 , and demonstrated that it plays important roles in both disease resistance and drought tolerance. Here, we conducted a promoter assay of Os2H16 and identified GT ‐1 as an important cis ‐element that mediates Os2H16 expression in response to pathogen attack and osmotic stress. Using the repeated GT ‐1 as bait, we characterized an abscisic acid, stress and ripening 2 ( ASR 2) protein from yeast‐one hybridization screening. Sequence alignments showed that the carboxy‐terminal domain of Os ASR 2 containing residues 80–138 was the DNA ‐binding domain. Furthermore, we identified that Os ASR 2 was specifically bound to GT ‐1 and activated the expression of the target gene Os2H16 , as well as GFP driven by the chimeric promoter of 2 × GT ‐1‐ 35S mini construct. Additionally, the expression of Os ASR 2 was elevated by pathogens and osmotic stress challenges. Overexpression of Os ASR 2 enhanced the resistance against Xanthomonas oryzae pv. oryzae and Rhizoctonia solani , and tolerance to drought in rice. These results suggest that the interaction between Os ASR 2 and GT ‐1 plays an important role in the crosstalk of the response of rice to biotic and abiotic stresses.