Open AccessOverexpression of Hevea brasiliensis ethylene response factor Hb ERF ‐ IX c5 enhances growth and tolerance to abiotic stress and affects laticifer differentiation
Author(s) -
Lestari Retno,
Rio Maryannick,
Martin Florence,
Leclercq Julie,
Woraathasin Natthakorn,
Roques Sandrine,
Dessailly Florence,
ClémentVidal Anne,
Sanier Christine,
Fabre Denis,
Melliti Sémi,
Suharsono Sony,
Montoro Pascal
Publication year - 2018
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12774
Subject(s) - hevea brasiliensis , biology , arabidopsis , methyl jasmonate , abiotic stress , jasmonate , transgene , microbiology and biotechnology , ethylene , hevea , arabidopsis thaliana , botany , gene , natural rubber , biochemistry , chemistry , organic chemistry , catalysis , mutant
Summary Ethylene response factor 1 ( ERF 1) is an essential integrator of the jasmonate and ethylene signalling pathways coordinating a large number of genes involved in plant defences. Its orthologue in Hevea brasiliensis , Hb ERF ‐ IX c5 , has been assumed to play a major role in laticifer metabolism and tolerance to harvesting stress for better latex production. This study sets out to establish and characterize rubber transgenic lines overexpressing Hb ERF ‐ IX c5 . Overexpression of Hb ERF ‐ IX c5 dramatically enhanced plant growth and enabled plants to maintain some ecophysiological parameters in response to abiotic stress such as water deficit, cold and salt treatments. This study revealed that Hb ERF ‐ IX c5 has rubber‐specific functions compared to Arabidopsis ERF 1 as transgenic plants overexpressing Hb ERF ‐ IX c5 accumulated more starch and differentiated more latex cells at the histological level. The role of Hb ERF ‐ IX c5 in driving the expression of some target genes involved in laticifer differentiation is discussed.