Open AccessMetagenomic‐based impact study of transgenic grapevine rootstock on its associated virome and soil bacteriome
Author(s) -
Hily JeanMichel,
Demanèche Sandrine,
Poulicard Nils,
Tannières Mélanie,
Djennane Samia,
Beuve Monique,
Vigne Emmanuelle,
Demangeat Gérard,
Komar Véronique,
Gertz Claude,
Marmonier Aurélie,
Hemmer Caroline,
Vigneron Sophie,
Marais Armelle,
Candresse Thierry,
Simonet Pascal,
Lemaire Olivier
Publication year - 2018
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12761
Subject(s) - biology , metagenomics , rootstock , biosafety , vineyard , horizontal gene transfer , human virome , transgene , genetically modified organism , microbiology and biotechnology , genome , genetically modified crops , genetics , gene , botany , horticulture
Summary For some crops, the only possible approach to gain a specific trait requires genome modification. The development of virus‐resistant transgenic plants based on the pathogen‐derived resistance strategy has been a success story for over three decades. However, potential risks associated with the technology, such as horizontal gene transfer ( HGT ) of any part of the transgene to an existing gene pool, have been raised. Here, we report no evidence of any undesirable impacts of genetically modified ( GM ) grapevine rootstock on its biotic environment. Using state of the art metagenomics, we analysed two compartments in depth, the targeted Grapevine fanleaf virus ( GFLV ) populations and nontargeted root‐associated microbiota. Our results reveal no statistically significant differences in the genetic diversity of bacteria that can be linked to the GM trait. In addition, no novel virus or bacteria recombinants of biosafety concern can be associated with transgenic grapevine rootstocks cultivated in commercial vineyard soil under greenhouse conditions for over 6 years.