Open AccessSuppression of OsMADS7 in rice endosperm stabilizes amylose content under high temperature stress
Author(s) -
Zhang Hua,
Xu Heng,
Feng Mengjie,
Zhu Ying
Publication year - 2018
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12745
Subject(s) - endosperm , amylose , biology , mads box , genetically modified rice , starch synthase , transgene , oryza sativa , gene , food science , botany , genetically modified crops , horticulture , agronomy , starch , biochemistry , arabidopsis , amylopectin , mutant
Summary High temperature significantly alters the amylose content of rice, resulting in mature grains with poor eating quality. However, only few genes and/or quantitative trait loci involved in this process have been isolated and the molecular mechanisms of this effect remain unclear. Here, we describe a floral organ identity gene, Os MADS 7 , involved in stabilizing rice amylose content at high temperature. Os MADS 7 is greatly induced by high temperature at the early filling stage. Constitutive suppression of Os MADS 7 stabilizes amylose content under high temperature stress but results in low spikelet fertility. However, rice plants with both stable amylose content at high temperature and normal spikelet fertility can be obtained by specifically suppressing Os MADS 7 in endosperm. GBSSI is the major enzyme responsible for amylose biosynthesis. A low filling rate and high expression of GBSSI were detected in Os MADS 7 RNA i plants at high temperature, which may be correlated with stabilized amylose content in these transgenic seeds under high temperature. Thus, specific suppression of Os MADS 7 in endosperm could improve the stability of rice amylose content at high temperature, and such transgenic materials may be a valuable genetic resource for breeding rice with elite thermal resilience.