Open AccessOs CESA 9 conserved‐site mutation leads to largely enhanced plant lodging resistance and biomass enzymatic saccharification by reducing cellulose DP and crystallinity in rice
Author(s) -
Li Fengcheng,
Xie Guosheng,
Huang Jiangfeng,
Zhang Ran,
Li Yu,
Zhang Miaomiao,
Wang Yanting,
Li Ao,
Li Xukai,
Xia Tao,
Qu Chengcheng,
Hu Fan,
Ragauskas Arthur J.,
Peng Liangcai
Publication year - 2017
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12700
Subject(s) - cellulose , biology , cell wall , mutant , biochemistry , cellulase , hydrolysis , oryza sativa , food science , gene
Summary Genetic modification of plant cell walls has been posed to reduce lignocellulose recalcitrance for enhancing biomass saccharification. Since cellulose synthase ( CESA ) gene was first identified, several dozen CESA mutants have been reported, but almost all mutants exhibit the defective phenotypes in plant growth and development. In this study, the rice ( Oryza sativa ) Osfc16 mutant with substitutions (W481C, P482S) at P‐ CR conserved site in CESA 9 shows a slightly affected plant growth and higher biomass yield by 25%–41% compared with wild type (Nipponbare, a japonica variety). Chemical and ultrastructural analyses indicate that Osfc16 has a significantly reduced cellulose crystallinity (CrI) and thinner secondary cell walls compared with wild type. CESA co‐ IP detection, together with implementations of a proteasome inhibitor ( MG 132) and two distinct cellulose inhibitors (Calcofluor, CGA ), shows that CESA 9 mutation could affect integrity of CESA4/7/9 complexes, which may lead to rapid CESA proteasome degradation for low‐ DP cellulose biosynthesis. These may reduce cellulose CrI, which improves plant lodging resistance, a major and integrated agronomic trait on plant growth and grain production, and enhances biomass enzymatic saccharification by up to 2.3‐fold and ethanol productivity by 34%–42%. This study has for the first time reported a direct modification for the low‐ DP cellulose production that has broad applications in biomass industries.