Open AccessSimultaneous stimulation of sedoheptulose 1,7‐bisphosphatase, fructose 1,6‐bisphophate aldolase and the photorespiratory glycine decarboxylase‐H protein increases CO 2 assimilation, vegetative biomass and seed yield in Arabidopsis
Author(s) -
Simkin Andrew J.,
LopezCalcagno Patricia E.,
Davey Philip A.,
Headland Lauren R.,
Lawson Tracy,
Timm Stefan,
Bauwe Hermann,
Raines Christine A.
Publication year - 2017
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12676
Subject(s) - biology , photosynthesis , fructose , fructose 1,6 bisphosphatase , biochemistry , aldolase a , glycine , transgene , botany , carboxy lyases , enzyme , gene , amino acid
Summary In this article, we have altered the levels of three different enzymes involved in the Calvin–Benson cycle and photorespiratory pathway. We have generated transgenic Arabidopsis plants with altered combinations of sedoheptulose 1,7‐bisphosphatase ( SBP ase), fructose 1,6‐bisphophate aldolase ( FBPA ) and the glycine decarboxylase‐H protein ( GDC ‐H) gene identified as targets to improve photosynthesis based on previous studies. Here, we show that increasing the levels of the three corresponding proteins, either independently or in combination, significantly increases the quantum efficiency of PSII . Furthermore, photosynthetic measurements demonstrated an increase in the maximum efficiency of CO 2 fixation in lines over‐expressing SBP ase and FBPA . Moreover, the co‐expression of GDC ‐H with SBP ase and FBPA resulted in a cumulative positive impact on leaf area and biomass. Finally, further analysis of transgenic lines revealed a cumulative increase of seed yield in SFH lines grown in high light. These results demonstrate the potential of multigene stacking for improving the productivity of food and energy crops.