Open AccessMain regulatory pathways, key genes and micro RNA s involved in flower formation and development of moso bamboo ( Phyllostachys edulis )
Author(s) -
Ge Wei,
Zhang Ying,
Cheng Zhanchao,
Hou Dan,
Li Xueping,
Gao Jian
Publication year - 2017
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12593
Subject(s) - biology , phyllostachys edulis , bamboo , microbiology and biotechnology , gene , signal transduction , gene expression , regulation of gene expression , rna , kegg , botany , computational biology , genetics , transcriptome
Summary Moso bamboo is characterized by infrequent sexual reproduction and erratic flowering habit; however, the molecular biology of flower formation and development is not well studied in this species. We studied the molecular regulation mechanisms of moso bamboo development and flowering by selecting three key regulatory pathways: plant–pathogen interaction, plant hormone signal transduction and protein processing in endoplasmic reticulum at different stages of flowering in moso bamboo. We selected PheDof1 , Phe MADS 14 and six micro RNA s involved in the three pathways through KEGG pathway and cluster analysis. Subcellular localization, transcriptional activation, Western blotting, in situ hybridization and qRT ‐ PCR were used to further investigate the expression patterns and regulatory roles of pivotal genes at different flower development stages. Differential expression patterns showed that PheDof1 , Phe MADS 14 and six mi RNA s may play vital regulatory roles in flower development and floral transition in moso bamboo. Our research paves way for further studies on metabolic regulatory networks and provides insight into the molecular regulation mechanisms of moso bamboo flowering and senescence.