Open AccessProteomic analysis of stress‐related proteins and metabolic pathways in Picea asperata somatic embryos during partial desiccation
Author(s) -
Jing Danlong,
Zhang Jianwei,
Xia Yan,
Kong Lisheng,
OuYang Fangqun,
Zhang Shougong,
Zhang Hanguo,
Wang Junhui
Publication year - 2017
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12588
Subject(s) - biology , abscisic acid , embryo , desiccation , somatic cell , desiccation tolerance , somatic embryogenesis , microbiology and biotechnology , endogeny , proteomics , metabolic pathway , biochemistry , cryptobiosis , botany , metabolism , embryogenesis , gene
Summary Partial desiccation treatment ( PDT ) stimulates germination and enhances the conversion of conifer somatic embryos. To better understand the mechanisms underlying the responses of somatic embryos to PDT , we used proteomic and physiological analyses to investigate these responses during PDT in Picea asperata . Comparative proteomic analysis revealed that, during PDT , stress‐related proteins were mainly involved in osmosis, endogenous hormones, antioxidative proteins, molecular chaperones and defence‐related proteins. Compared with those in cotyledonary embryos before PDT , these stress‐related proteins remained at high levels on days 7 (D7) and 14 (D14) of PDT . The proteins that differentially accumulated in the somatic embryos on D7 were mapped to stress and/or stimuli. They may also be involved in the glyoxylate cycle and the chitin metabolic process. The most significant difference in the differentially accumulated proteins occurred in the metabolic pathways of photosynthesis on D14. Furthermore, in accordance with the changes in stress‐related proteins, analyses of changes in water content, abscisic acid, indoleacetic acid and H 2 O 2 levels in the embryos indicated that PDT is involved in water‐deficit tolerance and affects endogenous hormones. Our results provide insight into the mechanisms responsible for the transition from morphologically mature to physiologically mature somatic embryos during the PDT process in P. asperata .