z-logo
open-access-imgOpen Access
Agronomic nitrogen‐use efficiency of rice can be increased by driving Os NRT 2.1 expression with the Os NAR 2.1 promoter
Author(s) -
Chen Jingguang,
Zhang Yong,
Tan Yawen,
Zhang Min,
Zhu Longlong,
Xu Guohua,
Fan Xiaorong
Publication year - 2016
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12531
Subject(s) - nitrogen , biology , promoter , oryza sativa , genetically modified rice , transgene , gene expression , gene , horticulture , microbiology and biotechnology , genetically modified crops , chemistry , genetics , organic chemistry
Summary The importance of the nitrate ( NO 3 − ) transporter for yield and nitrogen‐use efficiency ( NUE ) in rice was previously demonstrated using map‐based cloning. In this study, we enhanced the expression of the Os NRT 2.1 gene, which encodes a high‐affinity NO 3 − transporter, using a ubiquitin ( Ubi ) promoter and the NO 3 − ‐inducible promoter of the Os NAR 2.1 gene to drive Os NRT 2.1 expression in transgenic rice plants. Transgenic lines expressing pU bi:Os NRT 2.1 or pOsNAR 2.1:Os NRT 2.1 constructs exhibited the increased total biomass including yields of approximately 21% and 38% compared with wild‐type ( WT ) plants. The agricultural NUE ( ANUE ) of the pU bi:Os NRT 2.1 lines decreased to 83% of that of the WT plants, while the ANUE of the pOsNAR 2.1:Os NRT 2.1 lines increased to 128% of that of the WT plants. The dry matter transfer into grain decreased by 68% in the pU bi:Os NRT 2.1 lines and increased by 46% in the pOsNAR 2.1:Os NRT 2.1 lines relative to the WT . The expression of Os NRT 2.1 in shoot and grain showed that Ubi enhanced Os NRT 2.1 expression by 7.5‐fold averagely and Os NAR 2.1 promoters increased by about 80% higher than the WT . Interestingly, we found that the Os NAR 2.1 was expressed higher in all the organs of pU bi:Os NRT 2.1 lines; however, for pOsNAR 2.1:Os NRT 2.1 lines, Os NAR 2.1 expression was only increased in root, leaf sheaths and internodes. We show that increased expression of Os NRT 2.1 , especially driven by Os NAR 2.1 promoter, can improve the yield and NUE in rice.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here